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麦芽制造、酿酒和果汁业检测

MALTING, BREWING AND JUICE MANUFACTURE

Enzymes in the Brewing Industry - In beer manufacture, barley grain is malted (germinated under controlled conditions) and kilned. This malt is extracted to give wort. The wort is cooked and treated with hops, fermented with yeast, filtered and stabilised.

The aim of the malting process is to achieve maximum production of enzymes (particularly α-amylase and β-glucanase) and maximum depolymerisation of starch and β-glucan, with minimal respiration losses. Key enzyme activities are α-amylase, β-amylase, β-glucanase and limit-dextrinase. Xylanase may also be of some importance. In mashing, the aim is to produce maximum quantities of fermentable sugars in a liquor which is easy to filter.

The importance of β-glucan in affecting the rate of wort and beer filtration, and the potential for formation of β-glucan hazes in the final product, the beer, are well documented. The importance of arabinoxylan levels and their effect on filtration rates is less clear.

In the juice industry, enzymes are used to increase juice yield from the pulp, and are also used for juice clarification. Increased yields are obtained by depolymerization of the pectic matrix. However, this comes at a price. Released polymer fragments may self-associate to form hazes or precipitates e.g. arabinan hazes.

Analyte           Cat. No.        Analyte Significance Advantages of Megazyme Test Kits

α-Amylase

α-淀粉酶

K-CERA 麦芽质量的重要指标 反应快、试剂稳定。 AOAC 方法
2002.01; AACC 方法 22-02 

β-Amylase

β-淀粉酶 

K-BETA3 麦芽质量的重要指标 只有试剂盒可用。试剂稳定 

β-Glucan (Barley and oats)

β-葡聚糖(大麦和燕麦)

K-BGLU 大麦和燕麦的细胞壁多糖  反应快,试剂稳定,只有酶试剂盒可用。
AOAC 方法 995.16 

β-Glucanase

β-葡聚糖酶 

K-MBGL 麦芽中的b-葡聚糖酶水平  反应快,试剂稳定,只有酶试剂盒可用。 

D-Glucose

D-葡萄糖

K-GLUC
K-GLUHK
发酵混合物的主要成分  反应快、试剂稳定

Malt Amylase

麦芽淀粉酶

K-MALTA α-/β淀粉酶的测定。麦芽质量的主要指标 K-CERA和 K-BETA3的组合

Total Starch

总淀粉

K-TSTA
K-TSHK
粮食和饲料的淀粉含量  反应快、试剂稳定。AOAC 方法
996.11; AACC 方法 76-13; ICC 方法 No. 168 

α-Amylase

α-淀粉酶

T-AMZBG200 可测量收获前发了芽的大麦α-淀粉酶  方法新颖、反应快、试剂稳定 
 

Limit-Dextrinase

极限糊精

 K-PULLG6 水解关键酶 1,6-在淀粉和麦芽糊精联系和分支 新定义,可溶性底物。适用于自动分析仪及酶标仪系统。

β-Glucanase

β-葡聚糖酶 

T-BGZ200 水解β-葡聚糖的关键酶 方法新颖、反应快、试剂稳定 

Limit- Dextrinase

限制性糊精酶

T-LDZ200 水解淀粉和麦芽糊精的1-6交联  方法新颖、反应快、试剂稳定 

endo-β-Xylanase

β-木聚糖内切酶 

T-XAX200 水解麦芽木聚糖的关键酶  方法新颖、反应快、试剂稳定 
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